Claudio Marchisio has been ruled out for up to four weeks after suffering an injury setback on the same knee he had surgery on last year.
The 31-year-old underwent an operation on the anterior cruciate ligament in his left knee in April 2016, ruling him out of Italy's European Championship campaign, and required another procedure following complications.
Juve, who confirmed Marchisio's latest absence on Friday, revealed he required "specific therapies and muscle strengthening work", sidelining him for "the next three to four weeks".
It means Marchisio, who was substituted in last weekend's 3-0 Serie A win over Cagliari, will miss the forthcoming league matches against Genoa and Chievo, as well as the Champions League trip to Barcelona.
Marchisio this week seemingly ended speculation he could leave the Italian champions before the closure of the transfer window, with AC Milan and Chelsea having been linked.
"Sometimes, no words needed #nodoubt #thankyou," Marchisio wrote on Twitter in response to speculation about his future, alongside a photograph of him with his hand on the Juve crest.
-- Claudio Marchisio (@ClaMarchisio8) August 23, 2017