There were shocks in the first round of the Rosmalen Grass Court Championships as Timea Babos and Jelena Jankovic both lost in the first round.
Sixth seed Babos won the first set in 's-Hertogenbosch but fell victim to a fightback from wildcard Arantxa Rus, who recovered to win 5-7 6-2 6-3.
Former world number one Jankovic was stunned in straight sets by world number 277 Petra Krejsova, who prevailed 6-3 7-5.
Seventh seed Lesia Tsurenko beat Elise Mertens 6-4 6-1 while Camila Giorgi saw off Varvara Lepchenko in three sets.
At the Aegon Open in Nottingham, seventh seed Mona Barthel lost 6-3 5-7 7-5 to qualifier Jana Fett, while American Kristie Ahn carried through her momentum from qualifying by thumping eighth seed Naomi Osaka 6-1 6-1.
Elsewhere in the draw, there were wins for Yanina Wickmayer, Magda Linette, Hsieh Su-Wei, Tsvetana Pironkova and Julia Boserup.