London Welsh have been kicked out of the Championship and removed from the professional ranks after failing to meet the conditions to retain their licence.
The famous club, established way back in 1885, went into liquidation last month but were given a temporary licence to play their last two fixtures in the second tier.
However, that has now expired and the Exiles - who were only relegated from the Premiership in 2015 - have been unable to raise the funds to pay off their debts.
Rugby Football Union chairman Andy Cosslett said: "It is deeply regrettable that we should arrive at this point, but with London Welsh in liquidation and unable to satisfy conditions to compete in the Greene King IPA Championship as a professional club, participation in the competition became untenable,
"We know this will be an extremely disappointing day for everyone connected with London Welsh, including players, staff and supporters, and the sadness at the loss of this club from the professional ranks will be shared throughout the game."
There will be no relegation from the Championship in the absence of London Welsh, whose results will be expunged.