Chris Wood's Ryder Cup hopes hit a snag as he suffered a last-32 exit at the Paul Lawrie Match Play at the hands of Daniel Brooks.
Top seed Wood looked set to close in on a debut in the biennial competition against the United States in September following a comfortable first-round win over Brett Rumford, but his ambitions hit a setback against world number 444 Brooks.
Brooks raced into a six-hole lead on the front nine, but Wood showed great character to fight back following the turn.
The number one seed reduced the deficit by taking the 11th, 12th, 14th and 16th holes, but with nothing to separate them on 17, a 2&1 victory belonged to Brooks.
Brooks' will face Maximilian Kieffer in the last 16 after the German secured a two-hole victory over Mikko Ilonen with an eagle on the 18th - replicating Chris Paisley's feat in his win against Prom Meesawat.
Scottish Open champion Alex Noren - seeded second - overcame Renato Paratore 4&3.
Third seed Matthew Fitzpatrick kept his Ryder Cup aspirations on track by seeing off Matt Ford 6&5, but fourth seed Richard Bland was beaten 6&4 by Anthony Wall.
Richard Green fought from two down to force his match against host Paul Lawrie to an extra hole, where the Australian came out on top.
All players will play their last-16 matches on Friday due to concerns over the weekend's weather forecast.