World number five Simona Halep has withdrawn from the Rio Olympics due to concerns over the Zika virus.
The Romanian was listed as one of the entries for the Games by the ITF on Friday, before announcing she would not compete.
Halep, the 2014 French Open runner-up, joined Canadian Milos Raonic in withdrawing.
"I regret to announce that I have decided I will not compete in the Olympic Games taking place this summer in Rio," she wrote on Facebook.
"The reason for my decision is the concern regarding the danger posed by the Zika virus.
"After several talks with doctors and my family, I concluded that the risks are too high for my career and for my health, especially as a woman.
"Family is much too important for me and I can't risk not being able to have one of my own after my career in tennis is over.
"I know the Olympics are a very important event and I truly regret these circumstances. I hope the Romanian Olympic and Sports Committee and all Romanians will respect my decision. I wish all the participants, especially our delegation, the best of luck.
"I will continue to play, to proudly represent Romania in as many tournaments as possible, as I am doing right now at the BRD Bucharest Open."