Twilight Son's victory in the Diamond Jubilee Stakes completed a treble for Ryan Moore and Dartmouth gave the Queen her first win at Royal Ascot for three years on the final day of the meeting.
The prolific Moore chalked up a record nine wins in the week at the Berkshire Court last year and finished with six 12 months later to ensure he was the top jockey once again.
Moore was in the winners' enclosure once again after 7-2 shot Twilight Son stormed home to take the feature race by a neck on day five, giving trainer Henry Candy his first Royal Ascot win since 1979.
Gold-Fun was second in the six furlong sprint, with Signs Of Blessing third ahead of 3-1 favourite Magical Memory under Frankie Dettori in the only Group One on Saturday.
Dartmouth delivered a late birthday present for the Queen, who turned 90 last week, by staying on strongly to win the Hardwicke Stakes.
Her Majesty was present to witness her first Royal Ascot triumph since Estimate's famous Gold Cup success, with Olivier Peslier doing the steering aboard the four-year-old filly trained by Michael Stoute - who has now saddled a record 75 winners at the world-famous meeting.
Moore had started the day by riding odds-on favourite Churchill to victory in the Chesham Stakes over seven furlongs and combined with top trainer Aidan O'Brien yet again in the Wolferton Handicap to make it two out of two.
Outback Traveller showed great pace to win the Wokingham Stakes for Robert Cowley and Martin Harley at 10-1, while new father Adam Kirby took his fourth win of the festival on board 12/1 shot Commissioned in the last race of the day, the Queen Alexandra Stakes, for Gordon Elliott's yard.