Caroline Wozniacki was unable to claim a second victory on her return from injury as she gave up a one-set lead against Anett Kontaveit in round two of the Nottingham Open.
Wozniacki, who missed the entire clay-court season due to an ankle problem, recorded a straight-sets win over Cagla Buyukakcay on Tuesday, but was found wanting 24 hours on.
The former world number one claimed the first set against Kontaveit on a tie-break, only for the 20-year-old Estonian to hit back and triumph 6-7 (5-7) 6-3 7-5.
Kontaveit had led 4-1 in a dramatic decider, but was pegged back by the third seed before claiming another crucial break, to love, with the scores locked at 5-5.
While Wozniacki was eliminated, the two other seeds in action on Wednesday both claimed routine wins.
Top seed Karolina Pliskova was a 6-1 6-3 victor over Anna Tatishvili, while Monica Puig, the fourth seed, also dropped just four games - all in the first set - against Michelle Larcher De Brito.
The day's other match saw Alison Riske beat Hsieh Su-wei 6-2 6-4 to set up a quarter-final with Kontaveit.