It will start with nearly 2,000 rooms at seven hotels, including the 641 rooms at its flagship hotel in London's Covent Garden and sites at Gatwick Airport, Birmingham and Lincoln.
Travelodge, which has more than 500 hotels and 37,000 rooms in the UK, said one million man hours will go into the refit, along with 60,000 tins of paint and 30,000 new beds - enough to cover nine football pitches.
By the end of the year, Travelodge plans to have refitted more than 50% of its rooms, with 5,000 completed by the end of May alone.
By autumn 2014, almost 90% of Travelodge's estate will incorporate the new room design, which features a "sleep inducing" tranquil blue.
The refit, including a redesign of the chain's restaurants and public areas, is part of a £223 million investment plan to grow the business.
It comes after creditors backed a deal in September that allowed Travelodge to exit poorer performing leases while also freeing itself of a crippling debt burden.