Lulu and Sigma made guest appearances on the stage with Take That as the band performed to a crowd of thousands in London's Hyde Park.
The band belted out a mix of their classics and more recent hits at Barclaycard British Summer Time in the capital's famous park.
Lulu joined the three-piece for a rendition of their early 1990s hit Relight My Fire, followed with a performance by production duo Sigma of their latest collaboration Cry.
Olly Murs supported the chart-topping band and a clip of the former X Factor presenter singing Take That hit Pray was shared online.
Fans were treated to something a little different as the concert began with a performance of Nessun Dorma by an opera singer before the trio broke into Shine.
Gary Barlow, Mark Owen and Howard Donald sang their way through their back catalogue as flashy pyrotechnics drew cheers from the crowd.
Mark said he could not sleep with excitement as he looked forward to the concert. He told the 65,000 gig-goers: "Look at this, this is special. We are excited to be here. I haven't slept for three days."
The set was peppered with classics including 1993 hit Pray, during which the band performed their retro dance moves, Everything Changes and Back For Good.
Introducing their comeback song Patience, Howard said: "It's unbelievable that this next song was actually released 10 years ago."
Telling the crowd they are looking forward to their 25th anniversary next year, Gary thanked the familiar faces who have supported the band since they started out.
He said: "We really notice. We notice those faces who are always in the audience."
To a backdrop of fireworks and confetti they closed the show with Rule The World and Never Forget.