Ellie Goulding called for unity and asked people to "carry the spirit of Glastonbury home" as she performed at the festival.
The British singer told the crowd gathered at the Pyramid Stage that things had been strange following the vote in favour of leaving the European Union, but that she was happy to be in a place where people had come together.
Ellie - who earlier this week tweeted that she was "heartbroken" at the outcome of the referendum - said: "I know things have been a bit weird the past few days. Actually that's an understatement.
"But it is so nice to be in a place where there is so much unity and where everyone is so happy and friendly and dancing together and laughing together and loving each other."
She continued: "I really hope now for this lovely country that I live in and you live in that that sentiment, that spirit of Glastonbury, happens all over the country still, because I know some really terrible things have been happening and I really hope that when you go home that you guys will take that spirit with you.
"And if you see that that spirit is not happening wherever you live, wherever you are, to other people... We are all one person, we are all human beings.
"I just want to say I really hope that we can all carry the spirit of Glastonbury home with us, because that would make me very happy."
Ellie, 29, said she was honoured to be on the Pyramid Stage for the first time.
"It's very overwhelming and I am very very grateful to be here," she said.
The singer-songwriter performed a string of her hits during her one-hour set, including Burn and Anything Could Happen.