The price charged for cars using the M6 toll road has risen by more than 40p.
It means those driving during daytime weekday hours will be charged £5.90, while local tolls go up to £4.40.
The price has risen for car drivers following the takeover of the 27-mile stretch of privately owned road between Cannock and Coleshill in the West Midlands.
It will be the first price increase in more than five years. However, there is a freeze on HGV prices in a push for more lorries to use the road.
Bridget Fox, sustainable transport campaigner at the Campaign for Better Transport, spoke to the BBC. She said "It's a reminder of the failed experiment of the M6 Toll and the challenges faced by the new operators who are trying to balance the books".
"Developing toll roads in isolation has proved to be expensive and unpopular. Any road pricing should be part of a comprehensive scheme such as moving to a distance-based HGV levy."
The road was built in 2003 at a cost of more than £900 million and carries around 53,000 vehicles during the working week. Despite a freeze in prices for HGVs, however, Ed Neely – who owns Birmingham-based hauliers Neely Transport Ltd - claims it will make no difference.
"We don't use it at all because it's extortionate, it's £11 to drive six miles. If they made it free to HGVs we would use it. Until that time, it won't happen."