Barclays swung to a loss in the first quarter as further payment protection insurance (PPI) charges and a £1.4 billion settlement with the US Department of Justice knocked profits.
The UK bank reported a pre-tax loss of £236 million for the three months to March 31, having reported profits of £1.68 billion in the same period last year.
Stripped of litigation and conduct charges, Barclays said its attributable profit was £1.2 billion.
The bank was hit by a 2 billion US dollar (£1.4 billion) settlement reached with the US Department of Justice (DOJ) earlier this month, related to the sale of mortgage-backed securities in the lead-up to the financial crisis.
Chief executive Jes Staley said: "While the penalty was substantial, this settlement represents a major milestone for Barclays, putting behind us a significant, decade-old legacy matter."
Barclays booked an extra £400 million to cover PPI charges after seeing a higher number of complaints over the quarter.
While the charges affected Barclays' CET1 levels - referring to the capital cushion that underpins a bank's loans - Mr Staley said he was confident in the lender's position.
"This has been a significant quarter for Barclays, one in which we have shown that our new operating model and our portfolio of diversified, profitable businesses are capable of producing improved returns for shareholders."