Alesha Dixon and Anthony McPartlin will be seen wiping tears from their eyes during a schoolgirl's audition on Britain's Got Talent.
Jasmine Elcock, a 14-year-old student from Essex, also impresses Dixon's fellow judges Simon Cowell, David Walliams and Amanda Holden with her emotive rendition of Believe by Cher.
In Saturday's episode, viewers will see former Mis-Teeq singer Dixon dab her eyes with Walliams' handkerchief.
Perennially wisecracking host Ant is also clearly moved, while partner Dec is similarly wowed.
Speaking before her big moment on the stage, young Jasmine admits to nerves.
"I've performed in Annie in the West End at about the age of nine or 10, but I really get nervous," she is heard to say.
"My dad will always tell me to take three deep breaths. They're very supportive as parents. If I won Britain's Got Talent, it would be a dream come true."
The stakes are high as the winner of ITV's hit variety series will receive a cash prize of £250,000 and a slot on the Royal Variety Performance later this year.
Viewers will have to wait until Saturday's episode is broadcast to find out if Jasmine secured any Yeses from the judges.
In this instalment, Walliams' mother Kathleen makes an appearance on the panel as a last-minute replacement for Cowell, who was running late.
"I like nice male singers, preferably with their tops off," she says in the episode.
"Exactly what Simon likes as well!" her son quips in response.
Among the acts hoping to progress through to the next round are Sam, 14, and Hector, 12.
The acrobatic and gymnastics duo from Kent have been performing together for over two years.
Holden asks if they are related.
"We're partners, but we're not brothers," Hector replies.
"Not partners as in, like, going out. Gymnastics partners," Sam adds, to the amusement of the audience.
The young pair demonstrate strength and agility, but they have to win over the judging panel.
Vox Fortis are a classical vocal group comprising members Tom, Rodney, Cameron, Elias and Julias.
Ranging in age from 28-39, they have been together for just over six months.
Speaking before their audition, the group's Rodney says: "I'm really excited to be surrounded by these four very, very, very talented guys with a common interest.
"We want to be headed for that royal stamp of approval."
He will also be seen promising Cowell "a new sound" as Vox Fortis perform We Will Stand Together, based on Elgar's Nimrod, for their audition.
Other contestants hoping to win the panel's approval include the Ystrad Fawr Dancers, a Latin and ballroom dance troupe from Wales, and Ben Blaque, a crossbow performer from Missouri in the US.
The show will also feature Spartans Resurrection, a Scottish performance squad and The Blue Bag Lady from Hertfordshire, a woman who performs in a blue bag.
:: Britain's Got Talent is broadcast on ITV at 8pm.