Giorgio Chiellini has been replaced by Leonardo Bonucci in the Juventus squad to face Udinese in Serie A on Saturday after suffering a thigh strain.
Chiellini missed Italy's 2018 World Cup qualifying match against Macedonia in the week due to bruising on his right calf.
The 32-year-old centre-back suffered another muscle injury during a training session earlier on Saturday.
Bonucci, who had initially been handed the opportunity to rest by coach Massimiliano Allegri, has been called up as his replacement.
No timeframe has been given for Chiellini's return, with a Champions League encounter against Lyon on Tuesday followed by a meeting with AC Milan next weekend.