Usain Bolt's bid for history begins on Saturday, while Jessica Ennis-Hill will be hoping to retain her title as the heptathlon concludes.
Jamaican showman Bolt will take part in the 100 metre heats in the first athletics session of the day at the Olympic Stadium, with the crowd sure to be on the edge of their seats.
Ennis-Hill started her campaign on Friday and returns ahead of the rest of the pack with two events remaining, while fellow Briton Mo Farah will be looking to defend his 10,000m title.
In the pool Australia's Cate Campbell has one last chance to claim an individual medal in the women's 50m freestyle and Monica Puig is aiming to win Puerto Rico's first Olympic gold in the women's tennis singles.
TREBLE-TREBLE CHASE BEGINS
History beckons for Bolt, who starred at Beijing 2008 with gold medals in the 100m, 200m and 4x100m, delighting fans across the globe.
Bolt repeated the feat at London 2012 is now bidding to become the first track athlete to complete an unprecedented triple-triple.
Justin Gatlin will be hoping to throw a spanner in the works in the first event for the 100m world record holder, though.
FARAH STRIVING FOR GLORY
Bolt is not the only one with another multiple triumph in mind, with Farah hunting a double-double - a feat only achieved once in long-distance running.
Finn Lasse Viren is the only man to have successfully defended an Olympic 5000m and 10,000m track double when he triumphed at Munich 1972 and Montreal 1976, and the first leg of Farah's attempt takes place on Saturday.
Victory would also make the 33-year-old Britain's most successful track Olympian, with three gold medals unprecedented in his home nation.
FINAL STRETCH FOR HEPTATHLETES
Ennis-Hill takes a 72-point lead over Belgium's Nafissatou Thiam into the second and final day of the Heptathlon.
The 30-year-old put in a strong display on Friday, topping the 100m hurdles and finishing second and third in the 200m and high jump respectively.
However, having come 12th in the shot put her advantage has not been as big as it could have been, so she will be looking for strong performances in the long jump, javelin and, finally, the 800m.
PUIG ONE STEP FROM GOLD
In their history, Puerto Rico have only managed to claim eight Olympic medals - and never gold.
World number 34 Monica Puig has guaranteed their ninth overall by reaching the final of the women's singles, but she will be aiming for their first gold on Centre Court.
However, Australian Open champion and Wimbledon finalist Angelique Kerber stands in her way in what should be an entertaining match.
LAST-CHANCE SALOON FOR CAMPBELL
Campbell is the world record holder in the women's 100m freestyle, but she was only able to finish sixth in this year's final.
The 24-year-old Australian won gold in the 4x100m freestyle relay final on day one, but her only remaining chance of an individual medal comes in Saturday's 50m showpiece.
Campbell faces stiff competition from sister Bronte, Olympic record holder Ranomi Kromowidjojo and Simone Manuel - who topped the podium in the 100m event.
Also in the pool, Michael Phelps goes in the 4x100m medley relay in his final event at Rio 2016 and what may be his last appearance at the Olympics.