Jo-Wilfried Tsonga has withdrawn from the Internazionali d'Italia due to a groin injury.
The world number seven received a bye into the second round but will be unable to fulfil his fixture after sustaining an injury while practicing, with lucky loser Lucas Pouille taking his place in the draw.
Tsonga pulled out of the Estoril Open last month due to physical problems, and he admitted this latest setback is a concern with the French Open set to begin on May 22.
"I stretched my adductor this morning," said the Frenchman.
"I was sick when I left Madrid for two days, and I came here and I tried to play a little bit to see how it goes, and this morning I just stretched my adductor.
"It's not a good thing to be a little bit injured ahead of Roland Garros, but I hope to recover enough quickly to prepare as well as possible."