Louis Oosthuizen has withdrawn from contention to represent South Africa at this year's Olympic Games.
World number 12 Oosthuizen is his country's second-highest ranked player behind Branden Grace, who moved above the 2010 Masters champion courtesy of his RBC Heritage success last week.
Oosthuizen's decision not to travel to Rio 2016 means Grace will likely be joined by Charl Schwartzel in Brazil.
A statement from Oosthuizen's management company read: "Louis Oosthuizen has regretfully withdrawn from the team to represent South Africa at this summer's Olympic Games in Brazil.
"The 2010 Open Champion told his country's Olympic committee of his decision after long deliberations citing family and schedule issues."
Oosthuizen himself added: "I have always represented South Africa with pride so didn't make my decision without a great deal of thought.
"I would like to wish our golfers and all other athletes competing in Brazil all the very best for success."
Oosthuizen is the third major champion to pull out of the Games in recent weeks, following Vijay Singh and Adam Scott.