Youngsters Valentine Holmes and Cameron Munster will not be considered for State of Origin selection by Queensland in 2016 after breaking a curfew on an Emerging Maroons camp.
Cronulla Sharks full-back Holmes was reportedly arrested and fined by police after a late-night incident in Brisbane, while Melbourne Storm's Munster also broke a curfew imposed on the squad.
The former could yet be handed further sanctions, with an investigation into his behaviour having been opened by Queensland Rugby League (QRL) and the NRL's Integrity Unit.
In addition, four other players are reported to have broken curfew and may also face punishment.
A statement from the QRL on Monday read: "Managing director Robert Moore has today confirmed the QRL will exclude two players from its representative programs for the next 12 months.
"Cronulla Sharks player Valentine Holmes broke curfew during the Emerging Origin camp and is subject to further investigation by the QRL and NRL Integrity Unit.
"Melbourne Storm player Cameron Munster has been excluded solely for breaking curfew.
"Moore said it was possible that action would be taken against other players if they were identified as breaking curfew."
Kevin Walters, who was appointed as the Maroons' new coach in December, expressed disappointment at the behaviour of Holmes and Munster.
"On Saturday afternoon we sat down as a group and spoke about the importance of making the right choices," Walters said.
"Regrettably, there were some players who made poor choices after we had wrapped up the official part of that day's program."