England seam bowler James Anderson will miss the first Test against South Africa, which starts in Durban on Saturday, after failing to recover from a tight calf muscle in his right leg.
The 33-year-old quick managed to get through only five overs of the tour match against a South African Invitational XI in Potchefstroom this month.
He reported tightness in the calf muscle of his right leg on Monday and underwent scans on Tuesday.
Anderson, England's leading wicket-taker with 426 scalps in the long form of the game, will now have to wait to make his 111th Test appearance.
England's other pace bowlers on the tour, Stuart Broad, Steven Finn, Chris Jordan, Chris Woakes and Mark Footitt - the latter seeking to make his Test debut - are all available for the series opener at Kingsmead.