Paul Casey will not play in next year's Ryder Cup at Hazeltine after deciding against rejoining the European Tour next season.
The Englishman, who is largely based in Arizona, gave up his card ahead of 2015 and stated earlier this year that the decision to focus solely on the PGA Tour was the catalyst for his return to the world's top 25.
However, it also threw into doubt Casey's hopes of representing Europe against United States in the biennial tournament as players must feature in a certain amount of European Tour events.
Previously, players had to play in 13 events to be considered, but earlier this week the European Tour reduced the quota to five, outside of the majors and World Golf Championships tournaments.
Casey, though, has not been persuaded to change his mind, citing family reasons as his decision for committing to the PGA Tour.
"Having given the matter a great deal of thought, I have decided not to take up European Tour membership for the coming season," Casey said in a statement released to the media.
"With my wife and young son as my priority, I have decided to continue to concentrate on the PGA Tour which has worked well for us this past year as I have climbed back up the rankings.
"I am very sad not to have the chance to qualify for the 2016 European Ryder Cup team but I believe this decision will help me to be the best I can be both on and off the course and is the right decision for my family."
Casey was a member of the European team that triumphed in 2004 and 2006, as well as the 2008 defeat in Valhalla.
He was also controversially overlooked as a wildcard pick by 2010 captain Colin Montgomerie despite being ranked seventh in the world at the time.
Europe, who will be captained by Darren Clarke at Hazeltine in 2016, are aiming for a fourth consecutive Ryder Cup victory.