The Duke and Duchess and Cambridge will go on safari in India's Kaziranga National Park and learn how villagers live alongside threatened wildlife.
William and Kate will take a tour of the World Heritage Site which is a wildlife conservation area of global importance.
It is home to elephants, water buffalo, the endangered swamp deer, tigers, and two-thirds of the world's population of Indian one-horned rhinoceroses.
William has been campaigning for a number of years to protect endangered animals and on Tuesday park officials said another rhino had been poached, bringing the number killed in Kaziranga this year to six.
Poachers shot the rhinoceros and, while it was still alive, sawed off its horn before fleeing before dawn on Sunday, wildlife official Subasis Das said.
William and Kate planned a visit to Kaziranga specifically to focus global attention on conservation.
The visit coincides with the Bohag Bihu festival, the celebration of the Assamese new year, and on Tuesday evening around a campfire, the Duke and Duchess met local people and saw dance and musical performances.
During the day they will visit an agricultural village in the park and meet villagers in a community hall for a discussion about rural life in India, in a place where humans and animals live in close proximity.
In the afternoon they will visit the Centre for Wildlife Rehabilitation and Conservation which provides emergency care and rehab for wild animals that have been injured, displaced or orphaned.
After touring the centre they will visit the Kaziranga Discovery Park built by the Elephant Family, the charity founded by Mark Shand, the late brother of the Duchess of Cornwall, and see a first-of-its-kind health clinic for working elephants and an elephant information centre which is under construction.