Christopher Biggins said he was "very sorry and very sad" as he was removed from the Celebrity Big Brother house for saying that Aids was "a bisexual disease".
The entertainer, 67, was called into the diary room after a conversation with Mob Wives star Renee Graziano about how Aids originated and was told that it was his third use of "unacceptable language".
The first use was when he was discussing the Jewish community with Katie Waissel on Monday, and then when he told Renee in a separate conversation: "I think the worst type is the bisexuals, what it is is people not wanting to admit they are gay."
On Friday, Biggins said that homosexuals had been blamed for the rise of the "killer disease" but he thought it "was a bisexual disease, if I'm honest".
Renee said she had been told by an unnamed former CIA agent that "they gave it (Aids) to kill off third world countries," which Biggins agreed with, stating he thought it was "man-made".
Biggins then said, "There were a lot of bisexuals who went to those countries and had sex with those people and brought it back to their own families in America and that's how it became a worldwide disease."
Big Brother called him into the Diary Room later in the day and noted this comment was the third use of "unacceptable language", which left them with "no choice" but to remove him from the house.
"I'm very sorry and I'm very sad," said Biggins, who looked shocked as the charges were read out against him.
Biggins left the house immediately, and the housemates were called to the sofa by Big Brother to receive the shock news.
DJ James Whale opted to collect Biggins' suitcase from the storage room while the other housemates expressed their distress at the news.
EastEnders actor Ricky Norwood said a prayer for Biggins when he helped Whale pack up his belongings in the suitcase, while Sam Fox burst into tears.
Sam said: "I cant believe it, I'm upset."
Ricky reassured her with a hug, saying: "It's not on man, this is not right."
Viewers were divided over whether what Biggins said came under "free speech".
But others thought it was biphobic and Biggins deserved to leave.
It was pointed out that Big Brother's treatment of Biggins could be seen as hypocritical after Winston McKenzie was allowed to remain when he made homophobic comments last series.
Others wondered why Renee hadn't been warned by Big Brother too.
The news was followed by the eviction of Grant Bovey in the first public vote of the series, saving James.
After his exit, the housemates were asked to make an Eternal Nomination, which is a housemate who would be up for every eviction for the remainder of their time in the house.
Stephen Bear won the most nominations, causing an aggressive flare-up, where he screamed at those who voted for him not to speak to him for the rest of the series.